Intermediate

Part:BBa_K3440019:Design

Designed by: Julie Cordier, Victoria Muliadi   Group: iGEM20_Stockholm   (2020-10-23)


MtrB under Lux promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

Show that MtrB can be expressed under Plux in the presence of AHL


Source

LuxR from Vibrio fischeri pLux from Vibrio fischeri MtrB from Shewanella oneidensis

References